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Comparing the sugar fermentation assay and the gas production assay using liquid vs solid media.

http://technologyinscience.blogspot.com/2011/09/recombinant-protein-expression-in-ecoli.html

Recombinant Protein expression in E.coli, Best suitable strains for protein expression, advantages of using E.coli for choosing the host for protein expression.

0:00 Introduction
0:07 Protein Expression in E.coli
0:12 Advantages of E.coli System
1:45 E.Coli Strain & Application
2:23 Vectors for Protein Expression & Features
2:53 pUC19 Vector
3:08 Most widely used vectors
3:13 Cloning & Screening
3:26 Plasmid Extraction / Isolation
4:21 Restriction Digestion & Ligation
5:08 Restriction Digestion and Gel
5:15 Transformation and Expression
5:21 Transformation – Procedure
6:20 Shake Flask Culture- Protein Expression & Analysis
6:43 Lac Operon & IPTG Induction
7:43 Protein Analysis – SDS PAGE
8:01 Process Scale Up
8:08 Protein Characterization
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You can purchase Microbiology Books from the link given below
https://www.amazon.in/shop/microbiologywithsumi
Whenever a new micro-organism is discovered it becomes necessary to identify and catalogue it. For identification of this new microbe, it is first isolated and microscopically examined, and then further biochemically tested. Once these steps are carried out, we can identify this micro-organism. Biochemical tests are essential for the identification and classification of this micro-organism. Hence it is important to understand various biochemical tests and their functioning.
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“Bacteria can be divided into gram negative or gram positive depending on their cell wall/peptidoglycan make up. Gram positive implies thick peptidoglycan layer and stain a purple colour.

There are two main spp of gram positive cocci that causes human infections. These are Streptococci and staphylococci, they are differentiated by two main criteria: first their ability to produce the enzyme catalase. Streptococci are catalase negative. Staphylococci are catalase positive, which allow them to convert hydrogen peroxide to water and oxygen gas. Hence on a slide or tube bubbles are formed. Second criteria to differentiate staph and strep is based on microscopic appearance. Staphylococcus spp are usually in clusters, where as streptococci are arranged in pairs, or chains.

Streptococcal infections remain a serious health problem worldwide.
The streptococci are divided based on their oxygen requirement are classified into aerobes/facultative anaerobes and obligate anaerobes. Here we are focusing on the aerobic and facultative anaerobes which make up majority of strep spp. I am writing aerobic here because facultative anaerobes are able to survive in oxygen.

The Gram- positive streptococci are then further divided into the haemolytic properties in blood. The streptococcus spp. are smeared in blood agar which is a plate containing blood. Haemolytic property of the bacteria mean the bacteria ability to haemolyse, breakdown red blood cells. Streptococci are divided into three haemolytic groups:
· Alpha haemolytic
· Beta haemolytic
· Gamma haemolytic

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Streptococcus pyogenes – Microbiology for Medical Students

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