Recombinant Protein expression in E.coli, Best suitable strains for protein expression, advantages of using E.coli for choosing the host for protein expression.
0:00 Introduction
0:07 Protein Expression in E.coli
0:12 Advantages of E.coli System
1:45 E.Coli Strain & Application
2:23 Vectors for Protein Expression & Features
2:53 pUC19 Vector
3:08 Most widely used vectors
3:13 Cloning & Screening
3:26 Plasmid Extraction / Isolation
4:21 Restriction Digestion & Ligation
5:08 Restriction Digestion and Gel
5:15 Transformation and Expression
5:21 Transformation – Procedure
6:20 Shake Flask Culture- Protein Expression & Analysis
6:43 Lac Operon & IPTG Induction
7:43 Protein Analysis – SDS PAGE
8:01 Process Scale Up
8:08 Protein Characterization Video Rating: / 5
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Most people who want to get into better shape believe carbs are unhealthy. It is sad to see how many people believe that a carb-cutting diet is a path to rapid weight loss. Carbs are what the body needs for energy. Without carbs, you won’t be able to burn fat, and you’ll be depriving your body of the minerals and vitamins it requires to function properly. A balanced diet is all it takes to lose weight easily, quickly, and correctly. This video will discuss what are carbohydrates, and what types are best for your overall well-being.
What Are Carbs?
Carbs are the fiber, sugars, and starches contained in the food you eat. Much like fat and protein, carbohydrates are a macronutrient. Macronutrients are what gives the body energy, with carbs being the primary source. We must eat protein, fat, and carbs since our bodies can’t produce macronutrients on its own.
There are two types of carbohydrates: complex carbs and simple carbs.
Complex carbohydrates take a lengthy duration to process in the body. They help with the prolonged release of energy due to high amounts of nutrients and vitamins. Complex carbohydrates are beneficial when you’re trying to maintain a healthy lifestyle, as they provide the body with extra nutrition to use and last longer as a source of energy. Several examples of complex carbohydrates are brown rice, whole grains, wheat, oats, starchy vegetables, beans, peas, and lentils. Any kind of food containing these ingredients is much better for your body than their processed equivalent.
Simple carbohydrates are akin to processed food. They are regarded as simple sugars. Simple-carbs get separated in the body very fast and are used as an immediate energy source that burns out faster. They can be detrimental to the body when regularly consumed since they are comprised of refined sugars with minimal vitamins or nutrients. Some examples of simple carbohydrates include honey, sugar, fruit juices, milk, candy, soda, and biscuits.
Now that you know the difference between complex and simple carbs, let’s address the truly bad carbohydrates: refined-carbs.
If you are attempting to lose weight, you must reduce your consumption of refined carbohydrates. Refined carbs are the main cause of obesity and consist of high levels of fats, calories, and sugars. Processed or refined carbohydrates are germ and bran-less grains, and as such, have decreased nutrient content.
Refined carbs are found in foods made with white flour (cereals, pizza, pasta, bread, etc.) as well as white rice. Sugary beverages and potato chips are also high in refined carbs. Refined sugar is regarded as a refined carbohydrate. Therefore, ice creams, cookies, cakes, and donuts
are the most typical refined carb sources.
Integrate fresh vegetables, whole grains, fruits, and foods with fibre in your meals. They contain the proper carbs you should be feeding your body. Always choose the good sources of carbs, the good carbs can help you instead of the ones that will hurt you.
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Veggies, fruits, whole grains and dairy products are some of the best sources of healthy carbs.
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Whenever a new micro-organism is discovered it becomes necessary to identify and catalogue it. For identification of this new microbe, it is first isolated and microscopically examined, and then further biochemically tested. Once these steps are carried out, we can identify this micro-organism. Biochemical tests are essential for the identification and classification of this micro-organism. Hence it is important to understand various biochemical tests and their functioning. Video Rating: / 5
View full lesson: http://ed.ted.com/lessons/how-do-carbohydrates-impact-your-health-richard-j-wood
The things we eat and drink on a daily basis can impact our health in big ways. Too many carbohydrates, for instance, can lead to insulin resistance, which is a major contributor to cardiovascular disease and Type 2 Diabetes. But what are carbs, exactly? And what do they do to our bodies? Richard J. Wood explains.
Lesson by Richard J. Wood, animation by Qa’ed Mai.
Lesson on Vitamin C (Ascorbic acid), metabolic pathways that require vitamin C, dietary sources of vitamin C, and the physiology of absorption and metabolism of vitamin C. Vitamin C is an essential water-soluble vitamin that is required for several different biological pathways. Because it is essential, we have to get vitamin C from our diet. Vitamin C is important as a reducing agent and cofactor for several enzymes in biological pathways including collagen synthesis pathways. In the event of vitamin C deficiency, the condition known as Scurvy can occur and cause a wide range of signs and symptoms. Vitamin C is absorbed through our gastrointestinal system and metabolized and eliminated via several processes.
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Check out some of my other lessons.
Medical Terminology – The Basics – Lesson 1:
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**MEDICAL DISCLAIMER**: JJ Medicine does not provide medical advice, and the information available on this channel does not offer a diagnosis or advice regarding treatment. Information presented in these lessons is for educational purposes ONLY, and information presented here is not to be used as an alternative to a healthcare professional’s diagnosis and treatment of any person/animal.
Only a physician or other licensed healthcare professional are able to determine the requirement for medical assistance to be given to a patient. Please seek the advice of your physician or other licensed healthcare provider if you have any questions regarding a medical condition.
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Watch the next lesson: https://www.khanacademy.org/test-prep/mcat/chemical-processes/carbohydrates-5d/v/carbohydrates-naming-and-classification?utm_source=YT&utm_medium=Desc&utm_campaign=mcat
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MCAT on Khan Academy: Go ahead and practice some passage-based questions!
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This biology video tutorial provides a basic introduction into carbohydrates such as monosaccharides which include Glucose, Fructose, Galactose, and Ribose.
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Transferrin is a glycoprotein. Alcohol inhibits the glycosylation of several glycoproteins, including transferrin. Chronic alcoholism leads to transferrin deficient in four to five sialic acid residues.
Consumption of more than 60 g of alcohol/day leads to an increase in the plasma carbohydrate-deficient transferrin
concentration.
Other marker of alcoholic liver disease: γ-glutamyl transpeptidase. Video Rating: / 5
